Conducted by BIPSU
, Started on 2015 -
Completed on 2015
Completed
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The genus Rhodotorula have the ability to produce exopolysaccharide (EPS) a promising
compound known to have multifarious application in the industry. Plackett-Burman design
(PBD) was used to efficiently screen important medium composition, fermentation conditions
and highest producing Rhodotorula strains that would significantly increase EPS production.
Out of the seven variables, the most effective factors showing high level of significance are as
follows: the type of yeast preferring R. minuta BIOTECH 2178, high level of yeast extract and
xylose, low level of glucose and shorter fermentation time. Rhodotorula minuta BIOTECH
2178 was found to be a prolific EPS producer with a maximum production of 2.13+−0.48 g/L.
Thus, the experimental design used in this study provide a rapid identification of important
variables affecting EPS production. Subsequent optimization procedure of the significant
parameters can be further explored using response surface methodology.